To install this package, start R and enter:

## try http:// if https:// URLs are not supported
source("http://bioc.ism.ac.jp/biocLite.R")
biocLite("HSMMSingleCell")

In most cases, you don't need to download the package archive at all.

HSMMSingleCell

DOI: 10.18129/B9.bioc.HSMMSingleCell    

Single-cell RNA-Seq for differentiating human skeletal muscle myoblasts (HSMM)

Bioconductor version: Release (3.5)

Skeletal myoblasts undergo a well-characterized sequence of morphological and transcriptional changes during differentiation. In this experiment, primary human skeletal muscle myoblasts (HSMM) were expanded under high mitogen conditions (GM) and then differentiated by switching to low-mitogen media (DM). RNA-Seq libraries were sequenced from each of several hundred cells taken over a time-course of serum-induced differentiation. Between 49 and 77 cells were captured at each of four time points (0, 24, 48, 72 hours) following serum switch using the Fluidigm C1 microfluidic system. RNA from each cell was isolated and used to construct mRNA-Seq libraries, which were then sequenced to a depth of ~4 million reads per library, resulting in a complete gene expression profile for each cell.

Author: Cole Trapnell

Maintainer: Cole Trapnell <cole at broadinstitute.org>

Citation (from within R, enter citation("HSMMSingleCell")):

Installation

To install this package, start R and enter:

## try http:// if https:// URLs are not supported
source("http://bioc.ism.ac.jp/biocLite.R")
biocLite("HSMMSingleCell")

Documentation

PDF   Reference Manual

Details

biocViews ExperimentData, RNASeqData
Version 0.110.0
License Artistic-2.0
Depends R (>= 2.10)
Imports
LinkingTo
Suggests
SystemRequirements
Enhances
URL
Depends On Me
Imports Me monocle
Suggests Me cellTree
Build Report  

Package Archives

Follow Installation instructions to use this package in your R session.

Source Package HSMMSingleCell_0.110.0.tar.gz
Windows Binary
Mac OS X 10.11 (El Capitan)
Source Repository git clone https://git.bioconductor.org/packages/HSMMSingleCell
Package Short Url http://bioconductor.org/packages/HSMMSingleCell/
Package Downloads Report Download Stats

Documentation »

Bioconductor

R / CRAN packages and documentation

Support »

Please read the posting guide. Post questions about Bioconductor to one of the following locations: